Nano discovery inc manual#

Prepare libraries for Illumina sequencing in less than a day.

IDT for Illumina Unique Dual (UD) Indexes (24 or 96) offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell.

TruSeq DNA Nano with 96 CD Indexes supports 96-sample processing for high-throughput (HT) studies, and can be automated on liquid-handling robots (or processed manually).

Nano discovery inc manual#

TruSeq DNA Nano with Single Indexes supports 24-sample manual processing for low-throughput (LT) studies.

Truseq DNA Nano products are validated for high-quality genomic coverage for virtually any next-generation sequencing application. This enables researchers to access more information from each sequencing run. These kits also provide excellent coverage of traditionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. TruSeq DNA Nano reduces the number and average size of typical PCR-induced gaps in coverage. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. TruSeq DNA Nano offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp. TruSeq DNA Nano has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. Manual preparation of high-quality libraries in less than a day TruSeq DNA Nano enables efficient interrogation of samples with limited available DNA. Based on mechanical fragmentation–ligation chemistry, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.